• EI
  • Scopus
  • 中国科技期刊卓越行动计划项目资助期刊
  • 北大核心期刊
  • DOAJ
  • EBSCO
  • 中国核心学术期刊RCCSE A+
  • 中国精品科技期刊
  • JST China
  • FSTA
  • 中国农林核心期刊
  • 中国科技核心期刊CSTPCD
  • CA
  • WJCI
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
中国精品科技期刊2020
赵艳梅,鲁佳康,史玥玡,等. 乳酸对地衣芽孢杆菌HK高密度发酵的影响[J]. 华体会体育,2025,46(7):1−10. doi: 10.13386/j.issn1002-0306.2024050140.
引用本文: 赵艳梅,鲁佳康,史玥玡,等. 乳酸对地衣芽孢杆菌HK高密度发酵的影响[J]. 华体会体育,2025,46(7):1−10. doi: 10.13386/j.issn1002-0306.2024050140.
ZHAO Yanmei, LU Jiakang, SHI Yueya, et al. Influences of Lactate on the High-density Fermentation by Bacillus licheniformis HK[J]. Science and Technology of Food Industry, 2025, 46(7): 1−10. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024050140.
Citation: ZHAO Yanmei, LU Jiakang, SHI Yueya, et al. Influences of Lactate on the High-density Fermentation by Bacillus licheniformis HK[J]. Science and Technology of Food Industry, 2025, 46(7): 1−10. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024050140.

乳酸对地衣芽孢杆菌HK高密度发酵的影响

Influences of Lactate on the High-density Fermentation by Bacillus licheniformis HK

  • 摘要: 为了提高地衣芽孢杆菌HK(Bacillus licheniformis HK)的生物量和芽孢率,在20 L罐水平考察了发酵16~32 h耦合pH7.0流加乳酸(终浓度2.0%)对其生长代谢及产孢的影响,并基于转录组与代谢组差异分析了乳酸的代谢调控机制。结果表明:乳酸补料组的峰值生物量(30 h)为5.43×1010 CFU/mL,较对照(基础发酵组)提高54.3%;芽孢数为5.36×1010 CFU/mL,较对照提高60.0%。乳酸补料组的糖异生和磷酸戊糖途径关键基因ldhpckAgapBzwftkt较对照上调2.8~9.3倍;同时,其标志代谢物丙酮酸、6-磷酸果糖、1,3-二磷酸甘油酸、6-磷酸葡萄糖酸、5-磷酸-核酮糖、7-磷酸景天庚酮糖的丰度较对照增加1.55~12.6倍。TCA循环和氧化磷酸化关键基因citZicdcitBodhBatpABndhyumBqcrABCctaODCythAsdhAB表达较对照下调34.2%~96.8%而硝酸盐呼吸代谢基因nasBCD较对照上调107.4~287.3倍。与之相应的胞内关键代谢物顺乌头酸、异柠檬酸和柠檬酸丰度较对照降低52.6%~62.5%而NAD+、NADH丰度较对照增加1.6和2.7倍。同时,磷壁酸的前体3-磷酸甘油、肽聚糖的前体UDP-N-乙酰胞壁酸和UDP-N-乙酰胞壁酰-L-丙氨酸-D-谷氨酸的丰度较对照提高了1.5~14倍;而且,芽孢生成相关基因phrACcotPcotAyeekyheDtasAgerQyuzJ的表达较对照上调1.4~5.7倍而rapABabrByisIynzDspo0E较对照下调60.7%~96.7%。说明乳酸增强了糖异生、磷酸戊糖途径、硝酸盐呼吸、细胞壁合成,从而促进了细胞生长及芽孢的生成效率,为地衣芽孢杆菌高密度发酵生产提供了理论支撑。

     

    Abstract: To improve the biomass and spore rate of Bacillus licheniformis HK, influences of lactate on cell growth and metabolism and spore production were investigated at a 20 L bioreactor, and lactate addition was conducted by coupling with pH7.0 from 16 h to 32 h. Basing on the data differences in transcriptome and metabolome, the metabolic regulation mechanism of lactate was analyzed. The results showed that a peak biomass (30 h) of 5.43×1010 CFU/mL was obtained in lactate supplement group, which was 54.3% higher than that of basic fermentation group (control). Meanwhile, the spore number was 5.36×1010 CFU/mL, which was 60.0% higher than the control. Compared with the control, expressions of key genes in gluconeogenesis and pentose phosphate pathway (ldh, pckA, gapB, zwf, and tkt) were up-regulated by 2.8~9.3 fold in the lactate supplement group, the abundance of marker metabolites (pyruvate, fructose-6-phosphate, glyceric acid-1,3-diphosphate, 6-phosphate-gluconate, 5-phosphate-ribulose, and sedoheptulose 7-phosphate) in the pathways were increased by 1.55~12.6 fold compared with the control. Additionally, expressions of key genes in TCA cycle and oxidative phosphorylation system (citZ, icd, citB, odhB, atpAB, ndh, yumB, qcrABC, ctaODC, ythA, and sdhAB) were down-regulated by 34.2%~96.8%, while nitrate respiration metabolism gene (nasBCD) was up-regulated by 107.4~287.3 fold compared with the control. Abundances of corresponding key metabolites, such as aconitate, isocitrate and citrate, were decreased by 52.6%~62.5%, while the abundances of NAD+ and NADH were increased by 1.6~2.7 fold. Meanwhile, the precursors abundances of teichoic acid (glycerol-3-phosphate) and peptidoglycan (UDP-N-acetylmuramic acid, UDP-N-acetylmuramyl-L-alanine-D-glutamate) were increased by 1.5~14 fold compared with the control. Moreover, gene expressions involved in sporogenesis, such as phrAC, cotP, cotA, yeek, yheD, tasA, gerQ, yuzJ, were up-regulated by 1.4~5.7 fold, while rapAB, abrB, yisI, ynzD, and spo0E were down-regulated by 60.7%~96.7% compared with the control. The results suggested that lactate promoted gluconeogenesis, pentose phosphate pathway, nitrate respiration, and cell wall synthesis, thus promoting cell growth and spore formation efficiency. The results provided theoretical supports for high-density fermentation production of Bacillus licheniformis.

     

/

返回文章
返回